NTUIMCB_Eng

Recent Research Topics

Spindle scaling and orientation in normal and cancer cells

Synthetic polarity and asymmetric cell division in E. coli

Cellular reprogramming by small molecules

Laboratory: Laboratory of Systems and Synthetic Biology

Cell division and differentiation are among the most fundamental and fascinating processes in life. Perturbation of these processes can lead to cancerous proliferation and heterogeneity (or, beneficial survival for single-cell organisms). The lab uses mammalian and bacterial cells as our model systems; microscopy, small molecules, synthetic biology and computation as our primary approaches. We attempt to understand how governing molecules and quantitative scaling parameters are involved in symmetric/asymmetric divisions and differentiation, and how reconstruction may pave novel ways for therapies and applications.

Selected Research Publications

1.     Hong JC, Fan HC, Yang PJ, Lin DW, Wu HC and Huang HC*. (2021) Localized proteolysis for the construction of intracellular asymmetry in Escherichia coli. ACS Synthetic Biology 10, 1830-1836. (*Corresponding)

2.     Lin DW, Liu Y, Lee YQ, Yang PJ, Ho CT, Hong JC, Hsiao JC, Liao DC, Liang AJ, Hung TC, Chen YC, Tu HL, Hsu CP and Huang HC*. (2021) Construction of intracellular asymmetry and asymmetric division in Escherichia coli. Nature Communications 12, 888. (*Corresponding)

3.     Lai PL, Chen TC, Feng YC, Lin H, Chen Y, Wu N, Hsiao M, Lu J* and Huang HC*. (2020) Selection of a Malignant Subpopulation from a Colorectal Cancer Cell Line. Oncology Letters 20, 2937-2945. (*Corresponding)

4.     Tseng WC, Chen CK, Li JT, Sun M* and Huang HC*. (2019) Disentangling Experimental Noise from Fluorescent Microscopy Images with Multi-Cell Type. NeurIPS 2019 Workshop "Learning Meaningful Representations of Life". (*Corresponding)

5.     Chen Y, Nam S, Chaudhuri O* and Huang HC*. (2019) The evolution of spindles and their mechanical implications for cancer metastasis. Cell Cycle 18, 1671-1675. (*Corresponding) 

6.     Chen PC, Ho SY, Chen PL, Hung TC, Liang AJ, Kuo TF, Huang HC and Wang TA. (2017) Selective Targeting of Vibrios by Fluorescent Siderophore-Based Probes. ACS Chemical Biology 12, 2720-2724.

7.     Lai PL, Lin H, Chen SF, Yang SC, Hung SH, Chang CF, Chang HY, Lu FL, Lee YH, Liu YC, Huang HC* and Lu J*. (2017) Efficient Generation of Chemically Induced Mesenchymal Stem Cells from Human Dermal Fibroblasts. Scientific Reports 7, 44534. (*Corresponding)

8.     Young CF#, Tsai WY#, Chen WA#, Liang KW, Pan CJ, Lai PL, Yang PC and Huang HC*. (2016) Kinesin-5 Contributes to Spindle-length Scaling in the Evolution of Cancer toward Metastasis. Scientific Reports 6, 35767. (#Equal contribution) (*Corresponding)

9.     Wang WL, Huang HC, Kao SH, Hsu YC, Wang YT, Li KC, Chen YJ, Yu SL, Wang SP, Hsiao TH, Yang PC, Hong TM. (2015) Slug is temporally regulated by cyclin E in cell cycle and controls genomic stability. Oncogene 34, 1116-1125.

10.  Pan CJ and Huang HC*. (2013). Noise in Genetic Circuits: Hindrance or Chance? IEEE/ACM International Conference on Computer-Aided Design (ICCAD, review). (*Corresponding)

11.  Kwiatkowski N, Deng X, Wang J, Tan L, Villa F, Santaguida S, Huang HC, Mitchison T, Musacchio A, Gray N. (2012). Selective Aurora Kinase Inhibitors Identified Using a Taxol-Induced Checkpoint Sensitivity Screen. ACS Chemical Biology 7, 185-96.

12.  Shi J, Zhou I, Huang HC and Mitchison TJ. (2011). Navitoclax (ABT-263) accelerates apoptosis during drug-induced mitotic arrest by antagonizing Bcl-xL. Cancer Research 71, 4518-26.

13.  Huang HC, Mitchison TJ and Shi J. (2010). Stochastic competition between mechanistic independent slippage and death pathways determines cell fate during mitotic arrest. PLoS ONE 5, e15724.

14.  Huang HC*, Shi J, Orth JD and Mitchison TJ. (2010). Cell death when the SAC is out of commission. Cell Cycle 9, 2049-50. (*Corresponding)

15.  Huang HC*, Shi J, Orth JD and Mitchison TJ. (2009). Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly. Cancer Cell 16, 347-58. (*Corresponding)

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