NTUIMCB

近年研究主題

植物生物時鐘與晝夜節律之調控機制

研究室簡介

為適應地球自轉所產生的日夜週期變化，幾乎所有真核、原核生物皆依據日夜交替而有 ~24 小時的週期變化，生物學家將這樣的週期變化稱為「晝夜節律 (circadian rhythm) 」。植物在種子萌芽後，終其一生於萌芽處生長、發育並繁衍下一代，生長過程中除了可預期的日夜、四季的變化外，還會遭遇逢機多變的外在環境，然而植物個體無法遷徙躲避逆境，便觸發各種生理機制即時因應突發之環境變化。植物體內的「生物時鐘 (circadian clock) 」能讓生理途徑在遭遇非預期之短暫變化後，回歸與晝夜契合的節律而不紊亂，確保植物能在適切的季節進行世代交替。生物時鐘由一群相互回饋調控的基因所組成，各基因透過直接或間接的調控迴圈抑制或回饋活化上、下游基因表現，造成各個基因表現有規律的振盪並相互連動，基因表現的振盪則依據外在環境訊息如光、溫度的週期變化而調整振盪的週期與振幅，進而調控內在生理途徑運行的節律。已知模式植物阿拉伯芥中， LIGHT-REGULATED WD1 (LWD1) 為在黎明時活化生物時鐘運轉不可或缺的重要基因，其蛋白質中所具備的 5 個 WD 重複序列區域，使得 LWD1 具有提供蛋白質交互作用平台的特性，在本研究室先前所參與之研究中，已知 LWD1 能與特定的轉錄因子 TCPs 組成蛋白質複合體，直接活化生物時鐘核心基因 CCA1 ，確保曙光乍現時生物時鐘能正常運行。然而在缺乏 LWD1/LWD2 的突變株中，其他生物時鐘基因的表現形式所受的正、負影響卻無法只以調控迴圈的連動機制解釋，顯示植物生物時鐘的調控比目前已知的機制更為複雜。為深入瞭解植物生物時鐘調控的機制，我們利用 LWD1 尋找基因組中有類似轉錄表現形式的轉錄因子，藉由與 LWD1 共同表現之轉錄因子進一步釐清生物時鐘運行的分子機制，並進一步探討植物晝夜節律與生物時鐘如何調節植物生長發育與繁衍。 

Publications 

1.     Meng-Chun Lin, Huang-Lung Tsai, Sim-Lin Lim, Shih-Tong Jeng, and Shu-Hsing Wu (2017) Unraveling multifaceted contributions of small regulatory RNAs to photomorphogenic development in Arabidopsis. BMC Genomics 18: 559. (5-yr Impact factor: 4.284; Rank: 33/158 in Biotechnology & applied microbiology) 

2.     Jing-Fen Wu*, Huang-Lung Tsai*, Ignasius. Joanito, Yi-Chen Wu, Yi-Hang Li, Chin-Wen Chang, Jong-Chan Hong, Jhih-Wei Chu, Chao-Ping Hsu and S. H. Wu (2016) LWD-TCP complex activates the morning gene CCA1 in Arabidopsis. Nature Communications 7:13181 (*co-first authors)(5-yr Impact factor: 13.092; Rank: 3/64 in multidisciplinary sciences) 

3.     Huang-Lung Tsai, Yi-Hang Li, Wen-Ping Hsieh, Meng-Chun Lin, Ji Hoon Ahn, and Shu-Hsing Wu (2014) HUA ENHANCER1 is involved in posttranscriptional regulation of positive and negative regulators in Arabidopsis photomorphogenesis. The Plant Cell 26 (7): 2858-2872. (5-yr Impact factor: 9.975; Rank: 6/211 in Plant Sciences)(The work was selected as one of the significant publications in Academia Sinica 2014). 

4.     Ming-Jung Liu, Szu-Hsien Wu, Jing-Fen Wu, Wen-Dar Lin, Yi-Chen Wu, Tsung-Ying Tsai, Huang-Lung Tsai and Shu-Hsing Wu (2013) Translational landscape of photomorphogenic Arabidopsis. The Plant Cell 25 (10): 3699-3710. (5-yr Impact factor: 9.975; Rank: 6/211 in Plant Sciences) 

5.     Ya-Ling Lin, Shu-Chiun Sung, Hwang-Long Tsai, Ting-Ting Yu, Ramalingam Radjacommare, Raju Usharani, Antony S. Fatimababy, Hsia-Yin Lin, Ya-Ying Wang, and Hongyong Fu (2011) The defective proteasome but not substrate recognition function is responsible for the null phenotypes of the Arabidopsis proteasome subunit RPN10. The Plant Cell 23 (7): 2754-73. (5-yr Impact factor: 9.975; Rank: 6/211 in Plant Sciences) 

6.     Antony S. Fatimababy, Ya-Ling Lin, Raju Usharani, Ramalingam Radjacommare, Hsing-Ting Wang, Hwang-Long Tsai, Yenfen Lee and Hongyong Fu (2010) Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin/26S proteasome-mediated proteolysis. FEBS J. 277 (3): 796-816. (5-yr Impact factor 4.129; Rank: 82/286 in Biochemistry and Molecular Biology) 

7.     Huang-Lung Tsai, Wei-Ling Lue, Kuan-Jen Lu, Ming-Hsiun Hsieh, Shue-Mei Wang, and Jychian Chen (2009) Starch synthesis in Arabidopsis is achieved by spatial cotranscription of core starch metabolism genes. Plant Physiology 151 (3): 1582-1595. (5-yr Impact Factor: 7.428; Rank: 11/211 in Plant Sciences)

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